Innovative Methodology High-Resolution Multitransistor Array Recording of Electrical Field Potentials in Cultured Brain Slices

Hutzler, M., A. Lambacher, B. Eversmann, M. Jenkner, R. Thewes, and P. Fromherz. High-resolution multitransistor array recording of electrical field potentials in cultured brain slices. J Neurophysiol 96: 1638–1645, 2006. First published May 10, 2006; doi:10.1152/jn.00347.2006. We report on the recording of electrical activity in cultured hippocampal slices by a multitransistor array (MTA) with 16,384 elements. Time-resolved imaging is achieved with a resolution of 7.8 m on an area of 1 mm at 2 kHz. A read-out of fewer elements allows an enhanced time resolution. Individual transistor signals are caused by local evoked field potentials. They agree with micropipette measurements in amplitude and shape. The spatial continuity of the records provides time-resolved images of evoked field potentials and allows the detection of functional correlations over large distances. As examples, fast propagating waves of presynaptic action potentials are recorded as well as patterns of excitatory postsynaptic potentials across and along cornu ammonis.